Journal: Redox Biology
Article Title: KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism
doi: 10.1016/j.redox.2020.101622
Figure Lengend Snippet: Knockdown of KLF2 or ATG7 or BECN1 reduced autophagy-related molecules and OB differentiation-related markers. KLF2 or ATG7 or BECN1 was knocked down in DPSCs and cells were cultured in β-glycerophosphate plus l -ascorbic acid-containing medium. A. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of KLF2. B. MDC staining for detection of autophagy vesicles after knockdown of KLF2. C. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of ATG7. D. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of BECN1.
Article Snippet: The si ATG7 (AM16708), si BECN1 (4457298), negative control si RNA (AM4611), si KLF2 (4392420) and DEPC-Treated Water (AM9922) were purchased from Ambion.
Techniques: Cell Culture, Western Blot, Staining